Open Access Research

Effect of transient expression of fluorescent protein probes in synovial and myoblast cell lines

Seiji Shibasaki1*, Aika Fujita1, Chihiro Usui1, Sachiko Watanabe1, Sachie Kitano2, Hajime Sano2 and Tsuyoshi Iwasaki1

Author Affiliations

1 Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, 1-3-6 Minatojima, Chuo-ku, Kobe 650-8530, Japan

2 Department of Internal Medicine, Division of Rheumatology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, 663-8501, Japan

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SpringerPlus 2012, 1:36 doi:10.1186/2193-1801-1-36

Published: 22 October 2012

Abstract

Here, we investigate the appropriate fluorescent proteins for use in the culture of synovial MH7A and myoblast C2C12 cells. Fluorescent signal intensities of 3 different fluorescent proteins were examined in these cell lines. The fluorescent intensity of transiently expressed AcGFP, DsRed, and mStrawberry were examined in these cell lines, and the influence of the amount of plasmid used on transfection efficiency and cell viability were investigated.

Keywords:
C2C12 cell; Cell viability; Fluorescent protein; MH7A cell; Transfection