Open Access Research

Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity

Ahmed Ibrahim El-Batal1*, Abd-Algawad M Hashem2 and Noha M Abdelbaky1

Author Affiliations

1 Drug Radiation Research Department, National Center for Radiation Research & Technology (NCRRT), Nasr City, P.O. Box 29, Cairo, Egypt

2 Microbiology & Immunology Department, Faculty of Pharmacy, Cairo University, Kasr Al-Aini Street, P.O. Box 11562, Cairo, Egypt

For all author emails, please log on.

SpringerPlus 2013, 2:129  doi:10.1186/2193-1801-2-129

Published: 23 March 2013

Abstract

Aspergillus oryzae was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. Mobilized polyphenols were used as a green tool for synthesis and stabilization of gold nanoparticles (AuNPs). The radiation-induced AuNPs synthesis is a simple, clean and inexpensive process which involves radiolysis of aqueous solution that provides an efficient method to reduce metal ions. Gamma irradiated aqueous extract of fermented soybean and garlic was used for rapid preparation of AuNPs combining both effects of radiolytic reactions by radiation and stabilization by bioactive components of fermented extract. The synthesized AuNPs were confirmed by UV-Visible spectrophotometry, dynamic light scattering (DLS), Fourier Transform infra red (FT-IR) spectrophotometry, and transmission electron microscope (TEM) analysis which revealed morphology of spherical AuNPs with size ranging from 7–12 nm. The synthesized AuNPs exhibited antimicrobial activity against both Gram positive and Gram negative bacteria, as measured by well diffusion assay.

Keywords:
Solid state fermentation; Soybean; Garlic; Gamma radiation; Gold nanoparticles; Antimicrobial activity