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Open Access Research

The genetic study utility of a hexaploid wheat DH population with non-recombinant A- and B-genomes

Ming Hao1, Jixiang Chen1, Lianquan Zhang1*, Jiangtao Luo1, Zhongwei Yuan1, Zehong Yan1, Bo Zhang2, Wenjie Chen2, Yuming Wei1, Huaigang Zhang2, Youliang Zheng1 and Dengcai Liu12

Author Affiliations

1 Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, P.R. China

2 Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, P.R. China

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SpringerPlus 2013, 2:131  doi:10.1186/2193-1801-2-131

Published: 25 March 2013

Abstract

To study the D-genome of the wild wheat relative Aegilops tauschii Cosson at the hexaploid level, we developed a synthetic doubled-haploid (DH) hexaploid wheat population, SynDH3. This population was derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from a cross between Triticum turgidum ssp. dicoccon PI377655 and A. tauschii ssp. strangulata AS66 × ssp. tauschii AS87. SynDH3 is a diploidization-hexaploid DH population containing recombinant D chromosomes from two different A. tauschii genotypes, with A and B chromosomes from T. turgidum being homogenous across the entire population. Using this population, we constructed a genetic map. Of the 440 markers used to construct the map, 421 (96%) were assigned to 12 linkage groups; these included 346 Diversity Arrays Technology (DArT) and 75 simple sequence repeat (SSR) markers. The total map length of the seven D chromosomes spanned 916.27 cM, with an average length of 130.90 cM per chromosome and an average distance between markers of 3.47 cM. Seven segregation distortion regions were detected on seven linkage groups. Out of 50 markers shared with those on a common wheat map, 37 showed a consistent order. The utility of the diploidization-hexaploid DH population for mapping qualitative trait genes was confirmed using the dominant glaucousness-inhibiting gene W2I as an example.

Keywords:
Allopolyploid; Aegilops tauschii; Doubled-haploid; Segregation distortion