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Open Access Highly Accessed Research

Small molecules inhibit growth, viability and ergosterol biosynthesis in Candida albicans

Sandeep B Rajput and S Mohan Karuppayil*

Author Affiliations

DST-FIST and UGC-SAP Sponsored School of Life Sciences, SRTM University, Nanded, 431-606, M.S, India

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SpringerPlus 2013, 2:26  doi:10.1186/2193-1801-2-26

Published: 29 January 2013

Abstract

The aim of this work was to evaluate the anti-Candida efficacy of twenty five molecules of plant origin. Based on their MICs, effective molecules were categorized into four categories. Susceptibility testing of test compounds was carried out by standard methodology (M27-A2) as per CLSI guidelines. Minimum Fungicidal Concentration (MFC) was determined as the lowest concentration of drug killing 99.9% cells. Effect on sterol profile was evaluated by sterol quantitation method. Among the screened molecules, cinnamaldehyde, piperidine, citral, furfuraldehyde and indole were potent inhibitors of growth and viability. Exposure of Candida cells to cinnamaldehyde, piperidine, citral, furfuraldehyde, indole, α- and β- pinene at MIC’s, altered ergosterol profile. Our results indicate that the molecules altering sterol profile may exert their antifungal effect through inhibition of ergosterol biosynthesis and could be good candidates for fungal specific drug development.

Keywords:
Small molecules; Candida albicans; Ergosterol biosynthesis; Antifungals