Additional file 1.
Expression of musculoskeletal tissue marker genes in the head of crocodile embryos at stage 14. (A) Lateral view of the embryo. (B-D, and F) Frontal sections prepared around the plane indicated by the blue line in (A). (E) Frontal section prepared around the plane indicated by the red line in (A). (B) Expression of Runx2 is faintly detected at the mesenchymal cells above and below the eye, as well as in the mesenchyme distributed medial to the precursor of quadrate cartilage and in the mesenchyme surrounding the braincase (arrows). (C) Cranial muscular tissues are clearly labeled by MyoD probe. (D) Cartilaginous tissues, including the braincase and the quadrate (q), are labeled by Sox9 probe. (E) Scx is expressed in tendon precursor cells in close proximity of MyoD-positive jaw and eye muscle anlagen (arrowheads). (F) Six2 is expressed mainly in the mesenchyme around Sox9-positive cartilage precursors, including the quadrate and the braincase, as well as in the mesenchyme around MyoD-positive cranial muscle anlagen. The red outlined domains indicate the location of the anlagen of the jaw muscle complex. Scale bar in (A) is 1 mm. Scale bars in (B-F) are 0.5 mm.
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Tokita et al. SpringerPlus 2013 2:336 doi:10.1186/2193-1801-2-336