Additional file 2.

Expression of musculoskeletal tissue marker genes in the head of turtle embryos at stage 14. (A) Lateral view of the embryo. (B-E) Frontal sections prepared around the plane indicated by the red line in (A). (B) Runx2-positive mesenchymal cells are distributed above and below the eye, as well as in the domain medial to the anlagen of jaw adductor muscle (black arrows). (C) Cranial muscular tissues are clearly labeled by MyoD probe. (D) Cartilaginous tissues, including the braincase and quadrate, are labeled by Sox9 probe. (E) Six2 is expressed mainly in the mesenchyme around Sox9-positive cartilage precursors and MyoD-positive cranial muscle anlagen. Scale bar in (A) is 1 mm. Scale bars in (B-E) are 0.5 mm.

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Tokita et al. SpringerPlus 2013 2:336   doi:10.1186/2193-1801-2-336