Additional file 3.

A layer of fibrous connective tissue lateral to the external adductor muscle is found in late stage soft-shelled turtle embryo. (A) Lateral view of the head of a turtle embryo at stage 22. (B, C) Frontal sections of the head prepared in the planes indicated in (A). Note a clear layer of fibrous connective tissue lateral to the external adductor muscle (red arrowheads). (D) Lateral view of the head of a crocodile embryo at stage 20. (E, F) Frontal sections of the head prepared in the planes indicated in (D). (G) Lateral view of the head of a snake embryo at stage 31. (H, I) Frontal sections of the head prepared in the planes indicated in (G). A layer of fibrous connective tissue is not seen in the domain lateral to the external adductor muscle in crocodile and snake embryos. Rather, in crocodile and snake embryos, the domain is occupied by mesenchymal cells in low density or by acellular cavities. Scale bars are 1 mm.

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Tokita et al. SpringerPlus 2013 2:336   doi:10.1186/2193-1801-2-336