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Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera

Susheel Kumar1, Nutan Kaushik2* and Peter Proksch3

Author Affiliations

1 TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, India

2 The Energy and Resources Institute (TERI), Habitat Center, Lodhi Road, New Delhi, 110003, India

3 Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, 40225, Germany

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SpringerPlus 2013, 2:37  doi:10.1186/2193-1801-2-37

Published: 6 February 2013

Abstract

Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and λmax 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC50 value 35.4 μg/ml.

Keywords:
Chaetomium globosum; Endophyte; Withania somnifera; Antifungal; Sclerotinia