Open Access Technical note

Characterization of a novel polyclonal anti-hypusine antibody

Yurika Nishiki1, Thomas B Farb2, Jessica Friedrich2, Krister Bokvist2, Raghavendra G Mirmira13* and Bernhard Maier1

Author Affiliations

1 Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana, University School of Medicine, Indianapolis, IN 46202, USA

2 Lilly Research Labs, Eli Lilly & Co, Indianapolis, IN 46285, USA

3 Departments of Medicine, Cellular and Integrative Physiology, and Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA

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SpringerPlus 2013, 2:421  doi:10.1186/2193-1801-2-421

Published: 29 August 2013

Abstract

The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

Keywords:
Hypusine; eIF5A; Antibody; Recombinant proteins; Cell lines