SpringerOpen Newsletter

Receive periodic news and updates relating to SpringerOpen.

Open Access Short report

Genomic and expression analysis of a solute carrier protein (CcSLC25a5) gene from Cyprinus carpio Linnaeus

Li Jiang1*, Anda Cheng12, Yangyang Wang13 and Baoyong Zhang13

Author Affiliations

1 The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China

2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

3 College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China

For all author emails, please log on.

SpringerPlus 2013, 2:458  doi:10.1186/2193-1801-2-458

Published: 12 September 2013

Abstract

Using the Genefishing method, we identified seven potential regulatory genes involved in the process of scale morphogenesis in fishes. We further characterized a novel solute carrier protein gene (CcSLC), from the common carp which is differentially expressed in mirror carp and Jianli. The ORF encodes a peptide of 298 amino acids with a molecular mass of 31.5 kDa and a theoretical isoelectric point of 7.49. ScanProsite analysis indicated that it is a putative solute carrier protein that contains a substrate binding site. CcSLC was detected in carp embryos by in situ hybridization in the 70%-epiboly, 6-somite, and 14-somite embryonic stages. Gene expression stopped at the long pec stage. However, CcSLC25a5 was re-expressed during the initiation of scale formation in the regions that were scale covered. These findings provide novel insights into the features of early carp embryo and scale development.

Keywords:
Cyprinus carpio Linnaeus; Scale development; Solute carrier protein; Scale initiation; Embryo development