Genomic and expression analysis of a solute carrier protein (CcSLC25a5) gene from Cyprinus carpio Linnaeus
- Equal contributors
1 The Center for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences, Beijing 100141, China
2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
3 College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
SpringerPlus 2013, 2:458 doi:10.1186/2193-1801-2-458Published: 12 September 2013
Using the Genefishing method, we identified seven potential regulatory genes involved in the process of scale morphogenesis in fishes. We further characterized a novel solute carrier protein gene (CcSLC), from the common carp which is differentially expressed in mirror carp and Jianli. The ORF encodes a peptide of 298 amino acids with a molecular mass of 31.5 kDa and a theoretical isoelectric point of 7.49. ScanProsite analysis indicated that it is a putative solute carrier protein that contains a substrate binding site. CcSLC was detected in carp embryos by in situ hybridization in the 70%-epiboly, 6-somite, and 14-somite embryonic stages. Gene expression stopped at the long pec stage. However, CcSLC25a5 was re-expressed during the initiation of scale formation in the regions that were scale covered. These findings provide novel insights into the features of early carp embryo and scale development.