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Open Access Methodology

Resazurin-based assay for screening bacteria for radiation sensitivity

Deborah A Hudman and Neil J Sargentini*

Author Affiliations

Department of Microbiology and Immunology, AT Still University of Health Sciences, Kirksville, MO, USA

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SpringerPlus 2013, 2:55  doi:10.1186/2193-1801-2-55

Published: 16 February 2013

Abstract

We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.

Keywords:
Resazurin; Radiation-sensitivity assay; High throughput; X-radiation; UV radiation; Escherichia coli