Resazurin-based assay for screening bacteria for radiation sensitivity
Department of Microbiology and Immunology, AT Still University of Health Sciences, Kirksville, MO, USA
SpringerPlus 2013, 2:55 doi:10.1186/2193-1801-2-55Published: 16 February 2013
We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.