SpringerOpen Newsletter

Receive periodic news and updates relating to SpringerOpen.

Open Access Review

Bone marrow fibrosis as a feature of systemic lupus erythematosus: a case report and literature review

Émilie Chalayer1*, Martine Ffrench2 and Pascal Cathébras1

Author Affiliations

1 Service de médecine interne, Hôpital Nord, CHU de Saint-Étienne, Saint-Étienne, Cedex 2 42055, France

2 Laboratoire d'hématologie, Centre Hospitalier Lyon Sud, 165 Chemin du Grand Revoyet, 69495 Pierre Bénite, France

For all author emails, please log on.

SpringerPlus 2014, 3:349  doi:10.1186/2193-1801-3-349

The electronic version of this article is the complete one and can be found online at: http://www.springerplus.com/content/3/1/349


Received:20 June 2014
Accepted:26 June 2014
Published:9 July 2014

© 2014 Chalayer et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

Abstract

Introduction

Peripheral cytopenias are common in systemic lupus erythematosus (SLE), but bone marrow involvement is rarely reported. Myelofibrosis is a rare disorder characterized by reticulin fibrosis of the bone marrow, which usually occurs in response to clonal proliferation of hematopoietic stem cells in myeloproliferative disorders. However, bone marrow fibrosis has also been described in association with auto-immune diseases, especially SLE.

Method

We will report here a new case of bone marrow fibrosis associated with SLE. We also reviewed the 27 cases published in the English language literature, and will discuss the clinical presentation, outcome, treatment, and pathophysiology of bone marrow fibrosis occurring in association with SLE.

Results

Over one half of patients were diagnosed concomitantly with bone marrow fibrosis and SLE. Epidemiological, clinical and biological features of lupus were unremarkable. Except for the presence of reticulin fibrosis, the findings from the bone marrow biopsies proved highly variable. Overall mortality was about 14% but corticosteroid-based therapy lead to clinical improvement and reverted bone marrow fibrosis in most cases. Data on the usefulness of other immunomodulatory therapies are inconclusive.

Conclusions

SLE may be complicated by bone marrow involvement, of a likely autoimmune origin. Bone marrow fibrosis occurring with SLE is probably similar to “primary autoimmune myelofibrosis” and may respond to steroid and immunomodulatory therapies. Further studies with standardised proofreading of bone marrow aspirations and biopsies are needed to delineate the clinical and biological features of this rare complication of SLE.

Keywords:
Primary myelofibrosis; Bone marrow; Systemic lupus erythematosus

Introduction

Hematological abnormalities such as anemia, auto-immune hemolysis, leukopenia, lymphopenia and thrombocytopenia are very common in systemic lupus erythematosus (SLE) (Beyan et al. 2007). Blood cytopenias are among the criteria for SLE in the revised American College of Rheumatology (ACR) (Hochberg 1997) guidelines and may occur as the first symptom of the disease. Most of these manifestations are caused by increased peripheral destruction of blood cells associated with circulating auto-antibodies. Occasional case reports and small series have documented bone marrow abnormalities in patients with SLE, such as myelofibrosis, aplastic anemia, pure red cell aplasia, and features suggestive of myelodysplastic syndromes, suggesting that the bone marrow may also be a target organ in the disease. Bone marrow fibrosis is defined by the deposition of reticulin fibres in the bone marrow stroma. Fibrosis usually occurs in response to the clonal proliferation of hematopoietic stem cells (Kuter et al. 2007). Sporadic reports have suggested that bone marrow fibrosis may be part of the SLE disease spectrum, and the relationship between disease control and pancytopenia improvement provides indirect evidence for a causal relationship between SLE and bone marrow fibrosis. Auto-immune myelofibrosis may respond to immunosuppressive treatment with regression of the fibrosis and regeneration of the normal marrow tissue (Pullarkat et al. 2003). However, bone marrow involvement in SLE has not been thoroughly studied and the etiological mechanisms of this rare complication remain unclear. We report here a case of bone marrow fibrosis associated with SLE. In order to improve the knowledge on this rare but serious complication of SLE, we have also reviewed all previously published cases.

Case report

A 17 year-old woman with a history of polyarthralgias and photosentivity was referred to the hospital in 1999 for the evaluation of a Raynaud’s phenomenon. Clinical examination revealed no anomaly. She presented with neutropenia (leukocyte count 2.4 × 109/l with 1.1 × 109/l neutrophil granulocytes and 0.74 × 109/l lymphocytes) without anemia or thrombocytopenia. Antinuclear antibodies were present (>1/1280 with a speckled fluorescence pattern). Anti-DNA antibodies were absent. Anti-U1-RNP and anti-SSA antibodies were positive. Three years later (2002), she was admitted to the hospital because of atypical eating disorder and psychotic behaviour. No evidence for neuropsychiatric lupus was found despite an extensive work-up. The patient was diagnosed with schizophrenia and treated with olanzapine. Nine years later (2011), she was referred to the hospital for fatigue, fever and pancytopenia. With the exception of extreme paleness, clinical examination was unremarkable. Her weight is normal. Laboratory findings on admission showed the following values: leukocyte count 0,48 × 109/l with 0.28 × 109/l neutrophil granulocytes and 0.08 × 109/l lymphocytes, hemoglobin 2.9 g/dl, platelet count 15 × 109/l and reticulocyte count 3 × 109/l, low-normal level of folate, normal levels of vitamin B12 and ferritin. Anti-SSA, anti-RNP 70 and anti-U1-RNP antibodies were positive. The anti-DNA antibody titer was 76 UI/ml in ELISA [normal range < 10]. Direct Coombs’ test was negative. C3, C4, and CH50 were respectively 1.18 g/l [normal range: 0.79–1.52], 0.12 g/l [normal range: 0.2–0.51] and 120%. Abdominal ultrasonography showed limit homogen splenomegaly (around 12 cm). Tear drop cells or leukoerythroblastic blood smear were not noted. Two attempts to aspirate bone marrow at different sites were unsuccessful and yielded only dry taps. Bone marrow biopsy showed hypercellular marrow (cellularity: 80%) with focal lymphocytic infiltration, dysmyelopoiesis, erythrophagocytosis and grade 1–2 fibrosis. JAK-2, MPL W515L/K, and calreticulin mutations were not screen. A diagnosis of SLE with bone marrow involvement was retained. High dose methylprednisolone (500 mg/d) was given for 3 days. Then hydroxychloroquine and prednisone (1 mg/kg) were started, in addition to supportive care with blood transfusion and antibiotics. However, the patient remained pancytopenic. Intravenous immune globulins (30 g/d) were given for 4 days with remarkable improvement. Hemoglobin level after 2 months was 11.8 g/dl, platelet count was 175 × 109/l and leucocyte count was 3.27 × 109/l with 2.32 × 109/l neutrophil granulocytes and 0.49 × 109/l lymphocytes. Prednisone was continued for 17 months and gradually tapered. The patient did not experience change in her mental status during the course of steroid treatment. She remains in good physical health and still takes hydroxychloroquine and olanzapine.

Review of published cases

Method

An electronic search of the literature was performed via MEDLINE by crossing the key words “systemic lupus erythematosus” AND [“bone marrow fibrosis” OR “myelofibrosis”]. We then examined additional references from the retrieved articles. The study period ran from January 1975 through December 2013. Only papers written in English were reviewed.

Results

Clinical presentation (Table  1)

Table 1. Clinical presentation

Of the 27 cases retrieved from the English language literature and our case, 3 were males and 25 females, giving a ratio of 1:9. The age range was 12 to 70 years with a mean of 36 years and a median of 29 years. Fifteen patients received a concomitant diagnosis of SLE and bone marrow fibrosis, and 13 patients who had been previously diagnosed with SLE subsequently developed bone marrow fibrosis. In these 13 cases, the onset of bone marrow fibrosis varied from 8 months to 13 years after SLE diagnosis, with a mean of 5 years. Seven of these patients were under corticosteroids at the time of the hematological complication, two patients were under hydroxychloroquine, one had discontinued azathioprine at least 4 months before (Kiss et al. 2000) and one had started azathioprine two weeks before, but 4 weeks after discontinuing the drug no improvement had been observed (Vora et al. 1998). In patients with previously diagnosed SLE, symptoms attributed to the disease before bone marrow fibrosis occurred were rheumatologic symptoms (11/13), muco-cutaneous symptoms (8/13), renal involvement (3/13), serositis (3/13), and seizures (1/13). For all 28 patients, at the time of admission for cytopenias subsequently attributed to bone marrow fibrosis, the physical findings suggestive of SLE were rheumatologic symptoms (8/28), renal involvement (7/28), serositis (6/28) and muco-cutaneous symptoms (4/28). Splenomegaly was found in 11/26 patients (2 patients had undergone splenectomy due to immune thrombocytopenia), hepatomegaly in 11 patients, and lymph node enlargement or small diffuse lymph nodes in 6 patients. The other major symptoms reported were fever (17/28) and bleeding (15/28). Previous hematological history was often unclear, but at least 2 patients had been diagnosed with immune thrombocytopenia before the diagnosis of bone marrow fibrosis.

Peripheral hematological abnormalities (Table  2)

Table 2. Biology

Thirteen of the 28 patients had pancytopenia (anemia: Hb <10 g/dl, leukopenia: WBC < 4 × 109/l, and thrombocytopenia: platelets < 150 × 109/l), 13/28 had bicytopenia (anemia or/and leukopenia or/and thrombocytopenia), 1/28 had thrombocytopenia and neutropenia without leukopenia and 1/28, only thrombocytopenia. Neutropenia (<1,5 × 109/l) was observed in 10/12 (16 missing data) patients, lymphopenia in 4/9 (19 missing data), hemolysis with hyper-reticulocytosis in one case and a positive direct Coombs’ test in 10/18 (10 missing data). Hemoglobin levels ranged from 13.1 to 2.7 g/dl (mean 7.3 g/dl), platelets from 341 to 1 × 109/l (mean 50 × 109/l), and leucocytes from 6.8 to 0.35 × 109/l (mean 3.4 × 109/l). Of the 24 patients with thrombocytopenia, 11 had deep (<20 × 109/l), 8 severe (<50 × 109/l), and 5 moderate thrombocytopenia. Tear drop cells or leukoerythroblastic blood smear, two common finding in primary myelofibrosis, were noted in 14 patients.

Bone marrow abnormalities (Table  2)

For 22/28 patients, a “dry tap” occurred during bone marrow aspiration. All bone marrow biopsies showed bone marrow fibrosis with variable increases in the amount of reticulin fibers and fibroblasts. Grades of bone marrow fibrosis were not always specified. Global marrow cellularity was variable, ranging from increased (12/28) to normal (11/28) or decreased (5/28) without any increase in blasts. All elements including megakaryocytes appeared morphologically normal. Megakaryocytes were increased or/and with clustering in 13/28 patients and decreased in 1/28. Focal or massive lymphocytic infiltration was observed in 4/28, plasmocytosis in 1/28 and erythroid hyperplasia in 2/28. Fifteen patients underwent repeated bone marrow examination showing improvement, with reduction in reticulin amounts in 12/15.

Immunological abnormalities (Table  2)

Antinuclear antibodies were found in all patients, anti-dsDNA in 12/28, anti-Ro/SSA in 2/28, anti-histone in 1/28, and a low complement level in 18/28 patients. Antiphospholipid antibodies were detected in 4/28 patients. Anti-platelet antibodies testing came out positive in 3 patients and negative in 4.

Outcome and treatment (Table  3)

Table 3. Treatment

Follow-up time ranged from a few months to years but was often unspecified. The overall mortality was 14% (4/28). Two patients died within few days with no other treatment than blood transfusions and antibiotics. Two other patients who died received only prednisone as a specific treatment. Improvement was noted in 17/28 patients, transient response with need for new treatment in 5/28, and no improvement in 2/28. Supportive care with antibiotics and transfusions was explicitly mentioned for 8 patients. Two patients received granulocyte colony stimulating factor (G-CSF).

Immunomodulatory therapies consisted in corticosteroids (26/28) (prednisone, prednisolone or methylprednisolone), intravenous immune globulins (4/28) (Ramakrishna et al. 1995; Aharon et al. 1997; Sacre et al. 2009), plasma exchanges (2/28) (Borba et al. 1993; Vora et al. 1998), azathioprine (2/28) (Foley-Nolan et al. 1992; Kiss et al. 2000), cyclophosphamide (1/28) (Borba et al. 1993), cyclosporine (1/28) (Kiss et al. 2000), danazol (1/28) (Ramakrishna et al. 1995), colchicine (1/28) (Ramakrishna et al. 1995), vincristine (1/28) (Ramakrishna et al. 1995) and splenectomy (1/28) (Ramakrishna et al. 1995). Of 20 patients who received only corticosteroids, 16 improved and 4 did not. Four patients who received cortisone concomitantly with azathioprine, intravenous immunoglobulin or cyclosporine improved. One patient received cortisone and showed a transient response but pancytopenia relapsed so she received danazol, vincristine, colchicine, intravenous immunoglobulins, then underwent splenectomy and finally improved (Ramakrishna et al. 1995). Another patient was treated with plasma exchanges and cyclophosphamide following a transient response to cortisone and improved (Borba et al. 1993). One patient received intravenous immune globulins after 3 weeks of corticosteroid treatment without response, and a marked improvement occurred within the following week (Aharon et al. 1997).

Discussion

Nosology

Primary myelofibrosis is considered as a clonal myeloproliferative disorder (Tefferi et al. 2012). However some diseases such as infections, neoplasms and autoimmune diseases may also induce bone marrow fibrosis. The term “myelofibrosis” is used in some contexts to describe any increase in bone marrow stromal fibres, regardless of the associated disease, and in other contexts to define a specific myeloid disorder (primary myelofibrosis) (Kuter et al. 2007). The word “myelofibrosis” is therefore ambiguous, and in this article we have chosen rather to use the term “bone marrow fibrosis”. Some authors suggest the importance of distinguishing between increases in bone marrow reticulin and collagen. Above-normal reticulin amounts are generally regarded as a nonspecific sign of bone marrow abnormality, but may or may not be a sign of serious neoplastic disease. In contrast, increased collagen is less common and is mainly seen in tumours metastatic to the bone marrow or in the late stages of myeloproliferative diseases. Unlike increased reticulin, it is not always reversible (Kuter et al. 2007). In most cases reported here, it was unclear if trichrome collagen stain and/or reticulin stain were performed, and the type and amount of fibrosis were not reported according to established grading scales (Kuter et al. 2007). Another issue is whether finding bone marrow reticulin fibrosis per se should prompt a diagnosis of autoimmune myelofibrosis in a patient with SLE. For example, mild degrees of reticulin fibrosis can be observed in conditions such as immune thrombocytopenia and may be found in many patients with lupus when routine bone marrow biopsies are performed (Pereira et al. 1998). Moreover, some authors have reported cases of bone marrow fibrosis in patients who do not have SLE or other well-defined autoimmune syndromes (Bass et al. 2001; Pullarkat et al. 2003). They have defined “primary autoimmune myelofibrosis” as a disorder characterized by cytopenias with bone marrow lymphocyte infiltration and grade 3 - 4 reticulin fibrosis of the bone marrow, lack of atypical bone marrow cells or osteosclerosis, absent or mild splenomegaly, and the presence of auto-antibodies. In our review, the 28 retrieved cases have been considered as fulfilling criteria for SLE, although lupus symptoms and signs leading to the diagnosis of SLE were not always reported in detail by the authors. Their clinico-pathological features were very similar to those of the reported cases of “primary autoimmune myelofibrosis”. Thus we tend to believe that “autoimmune myelofibrosis”, just like autoimmune cytopenias, may occur as an isolated disorder, or as a feature of other autoimmune diseases including SLE. Finally, cases of aplastic anemia have also been reported in SLE patients. We found 25 published cases in the English language literature (Aplastic anemia as a feature of systemic lupus erythematosus. In preparation). In these cases, the bone marrow biopsy showed marked hypocellularity, but the absence of reticulin fibrosis was often not specified, and thus the differentiation between “lupus bone marrow fibrosis” and “lupus aplastic anemia” is not always clear, raising the question of the borderland between these two rare features of SLE (Cavalcant et al. 1978).

Pathophysiology

The pathogenesis of bone marrow fibrosis remains incompletely understood, but appears to be a relatively nonspecific response of fibroblasts to underlying cellular abnormalities. Increased reticulin is the result of fibroblast proliferation, and increased collagen synthesis or altered collagen turnover appear to be due to decreased collagenase release from macrophages and neutrophils (Kuter et al. 2007). Several growth factors appear to be implicated. The platelet-derived growth factor (PDGF), found in megakaryocytes and platelets, stimulates fibroblast growth (Kuter et al. 2007). The transforming growth factor β (TGFβ) and epidermal growth factor (EGF) are known to promote collagen synthesis (Le Bousse-Kerdilès et al. 2008). Immunological abnormalities may be involved in the pathogenesis. The increased circulating immune complexes and auto-antibodies that are present in SLE may act on megakaryocyte Fc receptors and release growth factors to promote marrow fibrosis. Some authors have suggested that both auto-antibodies against CD34+ stem cells and cytotoxic T cells may initiate and perpetuate damage to the bone marrow (Kiss et al. 2000). An increase in leucocyte apoptosis and impaired clearance of apoptotic cells has also been observed in patients with SLE. These apoptotic bodies were observed in the bone marrow of patients with SLE, while they are not typically seen in normal bone marrow. Delayed apoptotic cell clearance leads to prolonged exposure of auto-antigens and predisposes to antibody production (Hepburn et al. 2007). Furthermore, in the bone marrow of patients with bone marrow fibrosis and SLE, megakaryocyte counts are often above normal or normal. Therefore thrombocytopenia may result at least partly from an increased destruction of the platelets rather than a decreased production caused by bone marrow fibrosis. An association between immune thrombocytopenia (to which bone marrow dysfunction is increasingly believed to contribute (Gernsheimer 2009)) and bone marrow fibrosis has been observed in 3 of the 28 reported cases.

The JAK2 V617F mutation, associated with primary myeloproliferative disorders, is present in up to one half of the patients with primary myelofibrosis (Tefferi et al. 2012). Some authors suggest a thorough search for auto-immunity in the absence of the mutation (Sacre et al. 2009).

Clinical and biological presentation

Primary myelofibrosis is diagnosed relatively late in life (median age is 66 years) and is more common in males (ratio 3:2) (Tefferi et al. 2012). Bone marrow fibrosis occurring with SLE is diagnosed earlier (median age is 29 years) and is very uncommon in males (ratio 1:9). In 15/28 cases, the diagnosis of SLE and bone marrow fibrosis were made simultaneously. However, in 5 of these cases a number of symptoms and signs (such as arthralgias, alopecia, proteinuria) were suggestive of undiagnosed yet pre-existing SLE (Daly and Scott 1983; Matsouka et al. 1989; Inoue et al. 1992; Paquette et al. 1994; Pillai et al. 2009). Some authors suggest that autoimmune disorders, including SLE, may be considered in cases of bone marrow fibrosis in patients whose spleen is not enlarged (Pullarkat et al. 2003; Sacre et al. 2009), but 10/26 patients in our review had splenomegaly. Moreover, in primary myelofibrosis, the clinical finding of splenomegaly is associated with collagen, but not reticulin fibrosis (Thiele and Kvasnicka 2006). Fifteen patients underwent repeated bone marrow examinations showing improvement, with a reduction in reticulin in 12/15. This suggests that reticulin fibrosis (and maybe even collagen fibrosis) can be reversed if the underlying disease is treated (Pereira et al. 1998).

Outcome and treatment

Although it is likely that negative outcomes are less frequently reported in case reports, for which follow up data may be lacking, and that consequently the overall mortality may be higher than the 14% documented from this review, this mortality rate suggests a more favourable course for SLE-associated bone marrow fibrosis than for primary myelofibrosis (Tefferi et al. 2012). Interestingly, bone marrow fibrosis occurring with SLE appears to often respond to corticosteroids, unlike primary myelofibrosis. Plasma exchanges seem to have no efficacy. Intravenous immune globulins were used for 4 patients only (Ramakrishna et al. 1995; Aharon et al. 1997; Sacre et al. 2009), and proved to be efficient in at least one patient (Aharon et al. 1997), as it was the case for the patient we managed. None of the 28 patients received rituximab. None received an allogeneic hematopoietic stem cell transplant.

Conclusion

SLE may be complicated by bone marrow fibrosis, which is likely to be of autoimmune origin. This feature may be more common than previously thought, with cases being incorrectly characterized as blood peripheral cytopenias in patients previously diagnosed with SLE, and cases being misdiagnosed with primary myelofibrosis in patients not previously diagnosed with SLE. We think that in patients with SLE, cytopenias should be confirmed by bone marrow aspiration, and by bone marrow biopsy in atypical or refractory cases. Moreover, autoimmune myelofibrosis or SLE-associated bone marrow fibrosis should be considered in cases of primary myelofibrosis with atypical features such as young age and female sex, absence of spleen enlargement, or absence of JAK2 V617F mutation, because this condition seems amenable to efficient treatment. High-dose corticosteroid therapy with or without intravenous immune globulins should be the first-line therapy.

In order to improve knowledge of bone marrow involvement in SLE, we have established a French registry, with centralized proofreading of bone marrow aspirations and biopsies. We hope to achieve a sufficient sample size for epidemiological and clinical research on this unusual feature of lupus.

Consent

Written informed consent was obtained from the patient for the publication of this report.

Competing interests

The authors declare no conflict of interests.

Authors’ contributions

All authors participated and drafted the manuscript. All authors read and approved the final manuscript.

References

  • Agarwal BR, Bhalla K, Dalvi R, Currimbhoy ZE, Mehta KP (1995) Myelofibrosis secondary to SLE and its reversal on steroid therapy. Indian Pediatr 32:1207-1210 PubMed Abstract OpenURL

  • Aharon A, Levy Y, Bar-Dayan Y, Afek A, Zandman-Goddard G, Skurnik Y, Fabrrizzi F, Shoenfeld Y (1997) Successful treatment of early secondary myelofibrosis in SLE with IVIG. Lupus 6:408-411

    doi:10.1177/096120339700600412

    PubMed Abstract | Publisher Full Text OpenURL

  • Amital H, Rewald E, Levy Y, Bar-Dayan Y, Manthorpe R, Engervall P, Sherer Y, Langevitz P, Shoenfeld Y (2003) Fibrosis regression induced by intravenous gammaglobulin treatment. Ann Rheum Dis 62:175-177

    doi:10.1136/ard.62.2.175

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  • Aziz AR, Ashraf R, Mohammadian Y, Ruby C, Momin Z, Kumar A, Griciene P, Gintautas J (2004) Systemic lupus erythematosus presenting with pancytopenia due to bone marrow myelofibrosis in a 22-year-old male. Clin Adv Hematol Oncol 2:467-469

    discussion 469–470

    PubMed Abstract OpenURL

  • Bass RD, Randall D, Pullarkat V, Feinstein DI, Kaul A, Winberg CD, Brynes RK (2001) Pathology of autoimmune myelofibrosis a report of three cases and a review of the literature. Am J Clin Pathol 116:211-216 PubMed Abstract | Publisher Full Text OpenURL

  • Beyan E, Beyan C, Turan M (2007) Hematological presentation in systemic lupus erythematosus and its relationship with disease activity. Hematology 12:257-261

    doi:10.1080/10245330701214145

    PubMed Abstract | Publisher Full Text OpenURL

  • Borba EF, Pereira RM, Velloso ED, Pereira IA, Goncalves CR, Yoshinari NH (1993) Neutropenia associated with myelofibrosis in systemic lupus erythematosus. Acta Haematol 89:82-85 PubMed Abstract | Publisher Full Text OpenURL

  • Cavalcant J, Shadduck RK, Winkeistein A, Zeigler Z, Mendelow H (1978) Red-cell hypoplasia and increased bone marrow reticulin in systemic lupus erythematosis: Reversal with corticosteroid therapy. Am J Hematol 5:253-263 PubMed Abstract | Publisher Full Text OpenURL

  • Daly HM, Scott GL (1983) Myelofibrosis as a cause of pancytopenia in systemic lupus erythematosus. J Clin Pathol 36:1219-1222

    doi:10.1136/jcp.36.11.1219

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  • Durupt S, David G, Durieu I, Nove-Josserand R, Vital DD (2000) Myelofibrosis in systemic lupus erythematosus: a new case. Eur J Intern Med 11:98-100 PubMed Abstract | Publisher Full Text OpenURL

  • El Mouzan MI, Ahmad MA, al Fadel Saleh M, al Sohaibani MO, al Gindan YM (1988) Myelofibrosis and pancytopenia in systemic lupus erythematosus. Acta Haematol 80:219-221 PubMed Abstract | Publisher Full Text OpenURL

  • Foley-Nolan D, Martin MF, Rowbotham D, McVerry A, Gooi HC (1992) Systemic lupus erythematosus presenting with myelofibrosis. J Rheumatol 19:1303-1304 PubMed Abstract OpenURL

  • Gernsheimer T (2009) Chronic idiopathic thrombocytopenic purpura: mechanisms of pathogenesis. Oncologist 14:12-21

    doi:10.1634/theoncologist.2008-0132

    PubMed Abstract | Publisher Full Text OpenURL

  • Hepburn AL, Lampert IA, Boyle JJ, Horncastle D, Fai Ng W, Layton M, Vyse TJ, Botto M, Mason JC (2007) In vivo evidence for apoptosis in the bone marrow in systemic lupus erythematosus. Ann Rheum Dis 66:1106-1109

    doi:10.1136/ard.2006.065003

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  • Hirose W, Fukuya H, Anzai T, Kawagoe M, Kawai T, Watanabe K (1993) Myelofibrosis and systemic lupus erythematosus. J Rheumatol 20:2164-2166 PubMed Abstract OpenURL

  • Hochberg MC (1997) Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 40:1725-1734

    doi:10.1002/1529-0131(199709)40:9<1725::AID-ART29>3.0.CO;2-Y

    OpenURL

  • Inoue Y, Matsubara A, Okuya S, Okafuji K, Kaku K, Kaneko T (1992) Myelofibrosis and systemic lupus erythematosus: reversal of fibrosis with high-dose corticosteroid therapy. Acta Haematol 88:32-36 PubMed Abstract | Publisher Full Text OpenURL

  • Kaelin WG, Spivak JL (1986) Systemic lupus erythematosus and myelofibrosis. Am J Med 81:935-938 PubMed Abstract | Publisher Full Text OpenURL

  • Kiss E, Gaal IA, Simkovics EO, Kiss A (2000) Myelofibrosis in systemic lupus erythematosus. Leuk Lymphoma 39:661-665 PubMed Abstract | Publisher Full Text OpenURL

  • Kuter DJ, Bain B, Mufti G, Bagg A, Hasserjian RP (2007) Bone marrow fibrosis: pathophysiology and clinical significance of increased bone marrow stromal fibres. Br J Haematol 139:351-362 PubMed Abstract | Publisher Full Text OpenURL

  • Le Bousse-Kerdilès M-C, Martyré M-C, Samson M (2008) Cellular and molecular mechanisms underlying bone marrow and liver fibrosis: a review. Eur Cytokine Netw 19:69-80 PubMed Abstract | Publisher Full Text OpenURL

  • Matsouka C, Liouris J, Andrianakos A, Papademetriou C, Karvountzis G (1989) Systemic lupus erythematosus and myelofibrosis. Clin Rheumatol 8:402-407 PubMed Abstract | Publisher Full Text OpenURL

  • Nanji AA, Jetha N (1984) Myelofibrosis as a cause of pancytopenia in systemic lupus erythematosus. J Clin Pathol 37:714

    doi:10.1136/jcp.37.6.714-a

    OpenURL

  • Paquette RL, Meshkinpour A, Rosen PJ (1994) Autoimmune myelofibrosis. A steroid-responsive cause of bone marrow fibrosis associated with systemic lupus erythematosus. Medicine (Baltimore) 73:145-152 PubMed Abstract OpenURL

  • Pereira RMR, Velloso ERP, Menezes Y, Gualandro S, Vassalo J, Yoshinari NH (1998) Bone marrow findings in systemic lupus erythematosus patients with peripheral cytopenias. Clin Rheumatol 17:219-222 PubMed Abstract | Publisher Full Text OpenURL

  • Pillai A, Gautam M, Williamson H, Martlew V, Nash J, Thachil J (2009) Multisystem failure due to three coexisting autoimmune diseases. Intern Med 48:837-842 PubMed Abstract | Publisher Full Text OpenURL

  • Pullarkat V, Bass RD, Gong JZ, Feinstein DI, Brynes RK (2003) Primary autoimmune myelofibrosis: Definition of a distinct clinicopathologic syndrome. Am J Hematol 72:8-12

    doi:10.1002/ajh.10258

    PubMed Abstract | Publisher Full Text OpenURL

  • Ramakrishna R, Kyle PW, Day PJ, Manoharan A (1995) Evans’ syndrome, myelofibrosis and systemic lupus erythematosus: role of procollagens in myelofibrosis. Pathology 27:255-259 PubMed Abstract | Publisher Full Text OpenURL

  • Sacre K, Aguilar C, Deligny C, Choudat L, Koch P, Arfi S, Papo T (2009) Lytic bone lesions in lupus-associated myelofibrosis. Lupus 19:313-316

    doi:10.1177/0961203309349118

    PubMed Abstract | Publisher Full Text OpenURL

  • Sarkar RN, Banerjee S, Dey S, Saha A, Bhattacharjee P, Banerjee TK, Sinha PK, Chakraborty A (2009) Haematological presentation of systemic lupus erythematosus. J Assoc Physicians India 57:767-768 PubMed Abstract OpenURL

  • Tefferi A, Lasho TL, Jimma T, Finke CM, Gangat N, Vaidya R, Begna KH (2012) One Thousand Patients With Primary Myelofibrosis: The Mayo Clinic Experience. Mayo Clin Proc 87:25-33

    doi:10.1016/j.mayocp.2011.11.001

    PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  • Thiele J, Kvasnicka HM (2006) Grade of bone marrow fibrosis is associated with relevant hematological findings-a clinicopathological study on 865 patients with chronic idiopathic myelofibrosis. Ann Hematol 85:226-232

    doi:10.1007/s00277-005-0042-8

    PubMed Abstract | Publisher Full Text OpenURL

  • Vora BJ, Byers RJ, Lucas GS, Gokal R (1998) Reversal of osteomyelosclerosis-associated systemic lupus nephritis. Nephrol Dial Transplant 13:1559-1561

    doi:10.1093/ndt/13.6.1559

    PubMed Abstract | Publisher Full Text OpenURL