Research advances on structure and biological functions of integrins

Abstract

Integrins are an important family of adhesion molecules that were first discovered two decades ago. Integrins are transmembrane heterodimeric glycoprotein receptors consisting of α and β subunits, and are comprised of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Therein, integrin cytoplasmic domains may associate directly with numerous cytoskeletal proteins and intracellular signaling molecules, which are crucial for modulating fundamental cell processes and functions including cell adhesion, proliferation, migration, and survival. The purpose of this review is to describe the unique structure of each integrin subunit, primary cytoplasmic association proteins, and transduction signaling pathway of integrins, with an emphasis on their biological functions.

Background

Integrins are heterodimers consisting of two subunits. Hynes discovered there were 18 α and 8 β subunits forming 24 αβ heterodimers by noncovalent bonds (Hynes 2002). An electron microscope result showed that integrins have a globular head and two leg regions (one from α subunits and the other from β subunits) inserted into the plasma membrane, indicating each integrin subunit has an extracellular domain, a transmembrane domain, and a cytoplasmic tail (Srichai and Zent 2010). The α subunits mainly decide the type of ligands, and both α and β subunits are involved in cell signal transduction which are assisted by the contribution of adhesion molecules. The characteristics of integrin function and molecular diversity were initially clarified in 2000 (Zamir et al. 2000).

Based on the unique structure of integrins, including the α- and β-subunits, integrins can bind with extracellular matrix (ECM) proteins such as collagen (CO), laminin (LN), firbronection (FN), vitronectin (VN), and some other cellular receptors (Plow et al. 2000). The discovery of integrins at molecular level occurred in the late 1970s and 1980s, which was followed by further discoveries of integrin adhesion-related proteins, including structural protein members and signaling molecules (Rohrschneider 1980). Among these, the short intracellular cytoplasmic domains of integrins may associate directly with numerous cytoskeletal proteins and intracellular signaling molecules. These associated proteins provide a basis for modulating fundamental cell processes and various biological outcomes including proliferation, migration, cell differentiation, and apoptosis (Schwartz et al. 1995) by regulating signal transduction pathways. In recent years, many researchers have gradually developed a deep understanding of integrins using techniques like gene knockout, overexpression and specific antibodies. Meanwhile, researchers have also realized the crucial roles of integrins and make a greatly improved understanding for their unique structure, biological function, and integrin-mediated signal transduction mechanism in multiple cellular processes.

This review mainly focuses on the thorough understanding of the different subunit structural characteristics, biological functions, and associated proteins in cells.

Integrin structure and distribution

Integrin α subunits

The structures of different α subunits are very similar. The extracellular domains contain 7 homologous repeat domains with 30–40 amino acids, and the interval between these sequences has 20–30 amino acids. Extracellular domains also contain a ‘metal-ion-dependent adhesive site’ (MIDAS) that can bind divalent metal cations (Mg²⁺ or Ca²⁺) and is important in ligand binding. The transmembrane domains of integrins are single-spanning structures with 5 common amino acid sequences, ‘GFFKR’, its specific function is regulating integrin affinity by mediating an alpha–beta subunit cytoplasmic tail interaction. Cytoplasm domains of α subunits are generally short.

At least 18 α subunits have been found, including α1–α11, αD, αE, αL, αM, αV, αX, and αIIb. To date, their molecular structures have been studied using X-rays, nuclear magnetic resonance, electron microscopy, and three-dimensional ultrasonography. The components of extracellular domains include I-domain, β-Propeller, Thigh, Calf-1, and Calf-2 (Fig. 1). Nine different α subunits (α1, α2, α10, α11, αD, αL, αE, αM, αX) contain the I-domain structure, which is crucial for ligand binding sites. Several other α subunits (α3, α4, α5, α6, α7, α8, α9, αV, αIIb) contain no I-domain but constitute the ligand binding sites by β-Propeller. This article describes the characteristics of α subunit structures and tissue distributions in detail as shown in Table 1.

Fig. 1

Structure, primary cytoplasmic association proteins and biological functions of integrins

Table 1 Structural characteristics and tissue distributions of α subunits

Integrin β subunits

Integrin β subunits have an I-like domain similar to the I-domain in α subunits which is crucial for ligand binding. Other components include a plexin/semaphorin/integrin (PSI) domain, a hybrid domain, four epidermal growth factor (EGF) repeats, and a membrane proximal b tail domain (bTD), shown in Fig. 1. The β subunits also contain a large extracellular domain, a single-spanning transmembrane domain, and a short cytoplasmic tail (except for β4). The cytoplasmic domains lack catalytic activity themselves and are comprised of 60 amino acids (except for β4, which contains 1000 amino acids) (Hogervorst et al. 1990). Its cytoplasmic domains typically have two NP × Y sequences that provide binding sites to many proteins with phosphotyrosine-binding (PTB) domains (Bouaouina et al. 2008) and participate in cellular signal transduction by linking with cytoplasmic signal molecules (Gilcrease 2007). The super-family of integrin β can be divided into β1–β8 and their structural characteristics and tissue distributions are described in Table 2.

Table 2 Structural characteristics and tissue distributions of β subunits

Integrin-associated proteins

Integrin cytoplasmic domains associate directly with numerous cytoskeletal proteins and intracellular signaling molecules to modulate fundamental cell processes, as is shown in Fig. 1. Both α and β chains can participate in ligand binding specificity, but β chains alone seem to define cytoskeletal interactions.

The ability of integrin cytoplasmic domains may associate directly with several cytoskeletal proteins including α-actinin, talin, filamin, paxillin, and tensin (Reszka et al. 1992; Otey et al. 1993; Lyman et al. 1997; Geiger et al. 2001). Their binding sites and functions to integrins were summarized in Table 3.

Table 3 Integrin-associated proteins (cytoskeletal proteins)

In addition, integrin cytoplasmic domains may also interact directly with several intracellular signaling proteins such as cytohesin-1 (Kolanus et al. 1996), focal adhesion kinase (FAK) (Schaller et al. 1995), integrin-linked kinase (ILK) (Hannigan et al. 1996), β₃-endonexin (Shattil et al. 1995), cytoplasmic domain associated protein-1 (ICAP-1) (Chang et al. 1997), receptor for activated protein kinase C (Rack1) (Liliental and Chang 1998), and calcium- and integrin-binding protein (CIB) (Naik et al. 1997) (Table 4).

Table 4 Integrin-associated proteins (intracellular signaling proteins)

Biological functions and related signaling pathways

Integrins are responsible for sensing many aspects of the cellular microenvironment, including the composition and structure of the ECM and some biochemical signals generated by growth factor or cytokine stimulation. Integrins transmit bidirectional signaling across the plasma membrane by coupling extracellular conformational changes via the unclasping and separation of α and β transmembrane and cytoplasmic domains (Luo and Springer 2006). Inside-out signals regulate integrin affinity for adhesive ligands, outside-in signals depend on ligands that regulate cellular responses to adhesion (Ginsberg et al. 2005). Integrins have no intrinsic catalytic activities, and they transduce intracellular signals via adaptor proteins. Integration of these complex signals contributes to mediate cell biological processes (Parsons et al. 2010).

Integrins in cell adhesion

Integrin-mediated cell adhesion to extracellular matrix components is essential for the organization, maintenance, and repair of numerous tissues (De and Georges-Labouesse 2000). The cell adhesion process is complex and has a series of steps (Friedl and Wolf 2003), including binding to the extracellular matrix, receptor clustering, and the recruitment of cytoskeletal elements. Integrin-mediated cell adhesion occurs via focal adhesions involving the signaling pathway through ILK (serving as a multifunctional adaptor protein that links focal adhesion to the actin cytoskeleton (Hannigan et al. 2005), FAK, phospholipase C (PLC), and the activation of Pho family proteins. Therein, FAK modulates integrin activity (Lawson et al. 2012) and increases tyrosine phosphorylation in response to integrin activation depending on an intact integrin β cytoplasmic tail (Burridge et al. 1992). The Pho family proteins are important as well. Even the exact relationships between GTPase and integrin mediated-signal pathway are not clear, the integrin-dependent regulation of intracellular PH can occur by Pho GTPase, which has necessary effects on cell spreading and cell adhesion (Tominaga and Barber 1998). The signaling molecules involved in integrin-mediated adhesion are the upstream pathways that mediate other cell functions. Therefore, it is easy to see the link between cell adhesion and other integrin-mediated biological functions such as cell proliferation, survival, and migration. This may explain why integrin α5β1, after binding with FN and intracellular cytoskeletal components located in partial adhesion sites, can induce a series of signal transductions affecting cell motility and migration (Su et al. 2005). Kiwanuka et al. (2013) also indicated that α5β1, αVβ1, and αVβ6 integrin formed adhesions to provide points of traction for cell translocation during keratinocyte migration. Therefore, cell adhesion is the precondition of integrin-mediated biological functions.

Integrins in cell proliferation

Proliferation of mammalian cells is regulated by various environmental factors, primarily adhesion to ECM. Integrin-mediated adhesion and soluble factors are crucial to cell proliferation, the loss of cell adhesion leads to cell invasion and apoptosis (Blandin et al. 2016). A related study showed that integrins could be an indispensable player during intestinal tumorigenesis and serve as functional platforms to coordinate intestinal stem cell (ISC) maintenance, differentiation, and proliferation in response to environmental factors (Lin et al. 2013). The α2 and α3 subunits displayed an expression spatial gradient in the crypt and were implicated as cell growth patterns and phenotype modulators required for the process of intestinal epithelial cell differentiation (Zhang et al. 2003). Integrins also interact with growth factor receptors and other factors to regulate cell proliferation. Integrins and growth factor receptors can regulate G1 phase cyclins and related kinases that determine the cell cycle via various cytoplasmic signaling pathways (Moreno-Layseca and Streuli 2013; Eberwein et al. 2015).

There are many indications that not all integrin-mediated cell cycle signaling is the same. Most integrins activate FAK, extracellular regulated kinase (ERK), mitogen-activated protein kinases (MAPKs) and Rho family GTPases on rigid ECMs (Luo et al. 2013; Naci and Aoudjit 2014). However, integrin αvβ3 is selectively associated with enhanced signaling by RTK receptors. It can also activate several other pathways including calcium entry into cells (Schwartz and Denninghoff 1994), NF-ΚB (Scatena et al. 1998), and possibly some others. In addition, some integrins cannot induce similar effects despite their similar abilities at promoting cell adhesion and cytoskeletal organization. Integrins αvβ3, α5β1, and α1β1 interact with caveolin to stimulate Shc phosphorylation and possibly other factors to promote DNA synthesis (Wary et al. 1996). Integrins αvβ3 and α5β1 also activate PI3 K, which are phoaphatidylinositol lipids that modify enzymes implicated as mediators of integrin-mediated cytoskeletal changes and play an important role in cell migration (Cary et al. 1999).

Integrins in cell survival and migration

Cell migration is also vital to various biological phenomena. It is involved in not only normal but also pathological events. For example, cell migration is essential in homeostatic processes such as repairing injured tissues and body immune responses in adults (Steffensen et al. 2001). Cell adhesion receptors are essential for cell migration, and many belong to integrins (Liddington and Bankston 2000). A related report showed that integrin α4β7 had a high expression in mast leukocytes in mucosal inflammation, which promoted the migration of precursor cells to the intestinal tract. Meighan revealed that integrins expressions were up-regulated in migratory cells, and their activities were linked to cellular physiological differentiation (Meighan and Schwarzbauer 2008). During cell migration, integrins must have been recycled or synthesized.

Cell migration involves the localized activation of Rac for the directed protrusion of the cellular membrane only at the leading edges through both the ILK- and FAK-mediated pathways. The intracellular pH and calcium fluxes by integrins also affect cell migration (Schwartz et al. 1989; Marks et al. 1991).

Integrins also play a crucial role in cell survival and protect anchored cells against serum starvation-induced apoptosis. When epithelial and endothelial cell matrix attachment is disrupted, it induces cell apoptosis. Integrin-mediated cell survival is promoted by signaling through the PI3K-AKT, AKT, and ERK pathways (Naci and Aoudjit 2014). If cells are displaced or begin to migrate in an inappropriate environment, they will lose integrin-mediated survival signals (Gilcrease 2007). Signaling through AKT mediates cell survival in adherent epithelial cells by phosphorylating and sequestering BAD, which is a pro-apoptotic Bcl-2 family protein (Cory and Adams 2002). The signaling through the PI3 K-AKT pathway results in the phosphorylation of Bax, which is also a pro-apoptotic Bcl-2 family protein (Gilmore et al. 2000). Integrin-mediated signaling through the ERK pathway down-regulated the pro-apoptotic protein Bim (Reginato et al. 2003).

Conclusions and future prospects

Integrins have gradually become a research hotspot in cell biology, physiology, genetics, and pathology. Expression levels of integrins in cell membranes not only affect cell morphology, proliferation, differentiation, migration, and some macromolecular syntheses but also important in maintaining organization and structural integrity. According to the specific distribution and function of different integrin subunits, immense researchers have applied the unique structural and biological functions of integrins to study the prevention and treatment of human or animal diseases such as gastric cancer, liver cancer, and damaged tissues, et al. Additionally, integrins transmit bidirectional signaling to exert their biological functions, which plays an important role in cellular processes.

However, many questions remain to be elucidated, such as what the exact regulatory mechanisms are and how to determine integrin-mediated cell proliferation, migration, or survival in different cell or tissue types. Therefore, a better understanding of integrin characteristics and influences on human or animal functioning protein may provide a theoretical basis for clarifying the molecular mechanism of metastasis and solving these problems. The study of integrin-mediated signal transduction will also be an important area of research in the future.